yeast strain y187 (TaKaRa)
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Yeast Strain Y187, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/y187+yeast+strain/Y187+Yeast+Strain/10__1016_slash_j__jia__2026__04__018-87-12-15
Average 96 stars, based on 1258 article reviews
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Transformation Assay:Article Title: Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6. Article Snippet: The primary cilium, an immotile protrusion of vertebrate cells, detects chemical and mechanical stimuli in the extracellular milieu and transduces them into the cell body, thereby contributing to cellular development and homeostasis.. In the mammalian brain, serotonin receptor type 6 (Htr6) and other specific G protein-coupled receptors (GPCRs) localize preferentially to primary cilia and function in ciliary chemical detection; however, the molecular mechanism by which a subset of GPCRs is transported to primary cilia has not been fully elucidated.. In the present study, we demonstrate that a region in the fourth intracellular domain of Htr6 (Htr6 i4) is sufficient for ciliary localization. Article Title: Investigating the relationship Between AMBRA1 and Cell Proliferation in Melanoma Article Snippet: .. Yeast transformation, positive and negative control experiments were performed as stated by Yeast-Two Hybrid System manufacturer (Clontech Laboratories) a positive control mating was performed by mating Y2H gold strain fused with murine p53 and Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk Article Snippet: Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk. Article Snippet: Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Article Title: AT-HOOK MOTIF NUCLEAR LOCALIZED transcription factors AHL19, AHL21 and AHL23 - the modulators of the root system development. Article Snippet: AT-HOOK MOTIF NUCLEAR LOCALIZED (AHL) transcription factors regulate diverse developmental processes in plants, yet their roles in roots remain poorly understood.. Three closely related, co-expressed paralogs AHL19, AHL21, AHL23 display distinct expression patterns in Arabidopsis root tip; AHL19 and AHL23 are specifically active during lateral root (LR) primordia development.. Insertion mutants exhibit a markedly reduced root system (total length decreased by ~25–84 %), with additive effect in double and triple mutants. Article Title: XsMYB58 regulates XsLACS8 to promote the accumulation of seed oil in Yellowhorn. Article Snippet: An important source of nutrients and raw materials for humans, seed oils also provide energy for the post-germination growth of seeds.. Hence, enhancing seed oil content is crucial to improve overall oil yield.. Unsaturated fatty acids and rare neuronic acids are abundant in Yellowhorn (Xanthoceras sorbifolium Bunge) seed oil, which is used as a cooking oil or as a raw material to make biodiesel. Plasmid Preparation:Article Title: Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6. Article Snippet: The primary cilium, an immotile protrusion of vertebrate cells, detects chemical and mechanical stimuli in the extracellular milieu and transduces them into the cell body, thereby contributing to cellular development and homeostasis.. In the mammalian brain, serotonin receptor type 6 (Htr6) and other specific G protein-coupled receptors (GPCRs) localize preferentially to primary cilia and function in ciliary chemical detection; however, the molecular mechanism by which a subset of GPCRs is transported to primary cilia has not been fully elucidated.. In the present study, we demonstrate that a region in the fourth intracellular domain of Htr6 (Htr6 i4) is sufficient for ciliary localization. cDNA Library Assay:Article Title: Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6. Article Snippet: The primary cilium, an immotile protrusion of vertebrate cells, detects chemical and mechanical stimuli in the extracellular milieu and transduces them into the cell body, thereby contributing to cellular development and homeostasis.. In the mammalian brain, serotonin receptor type 6 (Htr6) and other specific G protein-coupled receptors (GPCRs) localize preferentially to primary cilia and function in ciliary chemical detection; however, the molecular mechanism by which a subset of GPCRs is transported to primary cilia has not been fully elucidated.. In the present study, we demonstrate that a region in the fourth intracellular domain of Htr6 (Htr6 i4) is sufficient for ciliary localization. Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk Article Snippet: Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Article Title: Pp6-Pfkfb1 axis modulates intracellular bacterial proliferation by orchestrating host-pathogen metabolic crosstalk. Article Snippet: Sequence encoding mouse Pp6 was inserted into the pGBKT7 vector (BD-Pp6) and transformed into Y2H Gold yeast strain using Yeastmaker Yeast Transformation System 2 (Clontech, Cat. 630440). .. A normalized universal mouse cDNA library transformed into Clone Assay:Article Title: Adaptor protein complex 1 facilitates ciliary localization of serotonin receptor type 6. Article Snippet: The primary cilium, an immotile protrusion of vertebrate cells, detects chemical and mechanical stimuli in the extracellular milieu and transduces them into the cell body, thereby contributing to cellular development and homeostasis.. In the mammalian brain, serotonin receptor type 6 (Htr6) and other specific G protein-coupled receptors (GPCRs) localize preferentially to primary cilia and function in ciliary chemical detection; however, the molecular mechanism by which a subset of GPCRs is transported to primary cilia has not been fully elucidated.. In the present study, we demonstrate that a region in the fourth intracellular domain of Htr6 (Htr6 i4) is sufficient for ciliary localization. Negative Control:Article Title: Investigating the relationship Between AMBRA1 and Cell Proliferation in Melanoma Article Snippet: .. Yeast transformation, positive and negative control experiments were performed as stated by Yeast-Two Hybrid System manufacturer (Clontech Laboratories) a positive control mating was performed by mating Y2H gold strain fused with murine p53 and Positive Control:Article Title: Investigating the relationship Between AMBRA1 and Cell Proliferation in Melanoma Article Snippet: .. Yeast transformation, positive and negative control experiments were performed as stated by Yeast-Two Hybrid System manufacturer (Clontech Laboratories) a positive control mating was performed by mating Y2H gold strain fused with murine p53 and Cell Culture:Article Title: Insect dome receptor directly recognizes viral envelopes to activate JAK/STAT signaling and induce conserved antiviral viperin expression. Article Snippet: E. coli BL21 (Biomed, Beijing, China; BC201), E. coli Rosetta (DE3) (Biomed, Beijing, China; BC204), E. coli DH10Bac (Thermo Fisher, 10361012) and Agrobacterium GV3101 (Biomed, Beijing, China; BC304) strains were cultured in Luria broth medium at 37◦C. .. Y2H Gold Yeast strain (Clontech, Mountain View, CA, USA; 630498) and |
![Yeast 2‐hybrid interaction assay of the interaction between (a) TrAP from ToLCNDV‐C ([C]), ToLCNDV‐T&C ([T&C]), ToLCKV‐T ([T]) and ToLCNDV‐C[M9] ([C‐M9]) and SlAGO1, CsAGO1, Slrgs‐CaM and SlATG7. (b) REn from ToLCNDV‐C, ToLCNDV‐T&C, ToLCKV‐T and ToLCNDV‐C[M9] and SlPolA, SlRBR, CsPCNA and SlPCNA. TrAP was expressed as a GAL4‐activating domain fusion protein in HGold yeast and mated with <t>Y187</t> yeast expressing the plant protein as a fusion protein to the GAL4‐DNA binding domain. The figure shows the mated yeast plated on triple dropout (−Trp/−Leu/−His) plates as selection for interaction. Empty vector controls are HGold expressing the empty vector pGADT7‐AD or Y187 expressing the empty pGBKT7‐BD vector.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8646/pmc12948646/pmc12948646__MPP-27-e70202-g007.jpg)